| 세션명 | 대학원생 구두발표 (II) |
|---|---|
| 발표장 | 제10회장 (105) |
| 논문코드 | 1O10-3 |
| 발표일 | 2018-10-11 |
| 발표시간 | 14:00-14:15 |
| 논문제목 | Protein Nanocapsules by Enzymatic Removal of a Polymer Template |
| 발표자 | 이채연 |
| 발표자 소속 | 부산대학교 |
| 저자 | 이채연, 황아란, Leeja Jose, 박지현1, 송재광1, 백현종† |
| 소속 | 부산대학교; 1한국화학연구원 |
| 논문초록 | Protein nanocapsules are potentially useful as functional nanocarriers because of their hollow structures, high biocompatibilies, and the intrinsic activity of their protein constituents. However, developing a facile method for the preparation of protein nanocapsules that retain their protein activity has been challenging. Here we describe the preparation of protein nanocapsules through the enzymatic removal of polymer templates. Nickel(II) nitrilotriacetic acid-end-functionalized poly(lactic acid) was introduced as a polymeric template to immobilize hexa-histidine-tagged green fluorescence proteins with consistent orientation. Following protein cross-linking and core-degradation, AFM and NMR study indicated the formation of protein hollow structures. We also confirmed the orientation control and the activity preservation of the protein after capsule preparation. |
THE POLYMER SOCIETY OF KOREA